8ng/ML H FABP Cardiac Marker Test Kit Heart Type Fatty Acid Test

A rapid test for the diagnosis of acute myocardial infarction (AMI) to detect Heart-Type Fatty Acid-Binding CE certified

Applications: 

The H-FABP Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid chromatographic immunoassay for the qualitative detection of human H-FABP in whole blood, serum or plasma as an aid in the diagnosis of acute myocardial infarction (AMI).

Description: 

FABP is a newly introduced plasma marker of acute myocardial infarction (AMI). The plasma kinetics of FABP (15 kD) closely resemble those of myoglobin in that elevated plasma concentrations are found within 2 hours after AMI and return to normal generally within 18 to 24 hours. But the concentration of FABP in the skeletal muscle is 20 times lower than in cardiac tissue (for myoglobin the same content for cardiac and skeletal tissue), that makes FABP to be more cardiac specific than myoglobin.

This makes FABP a useful biochemical marker for the early assessment or exclusion of AMI. FABP also appears to be a useful plasma marker for the estimation of myocardial infarct size. FABP is suitable for use as a standard in immunoassay for early detection of acute myocardial infarction, immunogen for antisera production, mass FABP standard, FABP biochemical and immunochemical studies, tracer for iodination.

How to use? 

Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.

1. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it within one hour.
2. Place the cassette on a clean and level surface.

For Serum or Plasma specimen:

• Hold the dropper vertically and transfer2 drops of serum or plasma (approximately 50mL) to the specimen area, then add 1 drops of buffer (approximately 40 mL), and start the timer. See illustration below.

For Venipuncture Whole Bloodspecimen:

• Hold the dropper vertically and transfer 2 drops of whole blood (approximately 50mL) to the specimen area, then add 1 drops of buffer (approximately 40 mL), and start the timer. See illustration below.

For Fingerstick Whole Bloodspecimen:

• To use a capillary tube: Fill the capillary tube and transfer approximately 50mL of fingerstick whole blood specimen to the specimen area of test cassette, then add 1 drop of buffer (approximately 40 mL) and start the timer. See illustration below.
• To use hanging drops: Allow 2 hanging drops of fingerstick whole blood specimen (approximately 50mL) to fall into the specimen area of test cassette, then add 1 drop of buffer (approximately 40 mL) and start the timer. See illustration below.

      3.Wait for the colored line(s) to appear. Read results at 10 minutes.  Do not interpret the result after 20 minutes.

INTERPRETATION OF RESULTS

(Please refer to the illustration above)

POSITIVE:*Two lines appear. One colored line should be in the control line region (C) and another apparent colored line should be in the test line region (T).

*NOTE: The intensity of the color in the test line region (T) will vary depending on the concentration of Fatty Acid-Binding Protein (H-FABP) present in the specimen. Therefore, any shade of color in the test line region (T) should be considered positive.

NEGATIVE: One colored line appears in the control line region (C). No line appears in the test line region (T).

INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test. If the problem persists, discontinue using the test kit immediately and contact your local distributor.

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