Serum Plasma CDV Ab Dog Test Kits , Canine Distemper Test Kit,Veterinary Rapid Test
The CDV Antibody Rapid Test Cassette (Serum/Plasma) is a lateral flow immunochromatographic assay for semi-quantitative
detection of Canine Distemper Virus Antibody in canine’s serum or plasma.
The CDV Antibody Rapid Test Cassette (Serum/Plasma) is based on sandwich lateral flow immunochromatographic assay. The test cassette has a testing window for the observation of assay running and result reading. The testing window has an invisible T(test) zone and a C (control) zone before running the assay.
When the treated sample was applied into the sample well on the cassette, the liquid will laterally flow through the surface of the test strip and react with the pre-coated CDV antigens. If there are anti-CDV antibodies in the specimen, a visible T line will appears.
The C line should always appear after a sample is applied, which indicates a valid result.
STORAGE AND STABILITY
Store as packaged in the sealed pouch either at room temperature or refrigerated (2-30 °C). The test cassette is stable through the expiration date printed on the sealed pouch. The test cassette must remain in the sealed pouch until use. DO NOT FREEZE. Do not store the test kit in direct sunlight.
Do not use after expiration date.
Handle all specimens as if they contain infectious agents.
Observe established precautions against microbiological hazards throughout testing and follow the standard procedures for proper disposal of specimens.
Wear disposable gloves and eye protection when specimens are being tested.
Humidity and temperature can adversely affect results.
Do not remove test cassette from its pouch until immediately before use.
Do not reuse the test kit.
Do not mix components from different lot and different products.
DIRECTIONS FOR USE
Allow the test cassette, specimen, buffer, and/or controls to equilibrate to room temperature (15-30 °C) prior to testing.
1.Remove the test cassette from the foil pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.
2. Collect canine’s fresh whole blood, and separate serum or plasma from blood as soon as possible to avoid hemolysis.
Only use clear, non-hemolyzed specimens. Do not leave the specimens at room temperature for prolonged periods.
Serum and plasma specimens may be stored at 2-8 °C for up to 3 days. For long term storage, specimens should be kept below -20 °C.
3.Place the test cassette on a clean and level surface. Hold the dropper vertically and transfer 1 drop of serum or plasma (approximately 40 μl) and immediately add 2 drops of buffer (approximately 80 μl) into the specimen well (S), then start the timer. See illustration below.
4. Read the results in 10 minutes by comparing the T line intensity with the color card provided. Do not interpret results after 15 minutes.
INTERPRETATION OF RESULTS
The presence of “C” line indicates a valid result. Read the result by comparing the color intensity of the “T” line with the standard color card provided. The V.N. titers of canine distemper antibodies are corresponding with the color intensity grades.
Positive: The presence of both “C” line and “T” line. T line at G3 is considered as standard positive. T line < G3, is weak
positive and indicates low CDV antibody level. T line around G4- G5, is strong positive. T line > G6, is very strong positive, indicates a contact with virus recently or in infection.
Negative: Clear C line appears. No T line appears, or T line is weaker than G1. It indicates very low CDV titer. Failed immunization may happen.
Invalid: No line appears in control line area(C).