Convenient Procalcitonin (PCT) Diagnostic Test Kit Use By Citest fluorescence Immunoassay In Whole Blood/Serum /Plasma
A test for measuring Procalcitonin (PCT) in whole blood /serum /plasma with the use of Citest TM fluorescence Immunoassay Analyzer. For professional in vitro diagnostic use only.
The PCT Test Cassette (Whole Blood/Serum/Plasma) is based on Fluorescence immunoassay for the quantitative detection of human Procalcitonin in whole blood, serum or plasma as an aid in the diagnosis of inflammatory conditions.
Procalcitonin (PCT) is a small protein that comprises 116 amino acid residues with a molecular weight of approximately 13 kDa which was first described by Moullec et al. in 1984. PCT is produced normally in C-cells of the thyroid glands. In 1993, the elevated level of PCT in patients with systemic infection of bacterial origin was reported and PCT is now considered to be the main marker of disorders accompanied by systemic inflammation and sepsis.
The diagnostic value of PCT is important due to the close correlation between PCT concentration and the severity of inflammation. It was shown that “inflammatory” PCT is not produced in C-cells. Cells of neuroendocrine origin are presumably the source of PCT during inflammation.
The test deviation is≤±15%.
2. Assay Range and Detection Limit
Assay Range: 0.1 – 50 ng/mL
Minimum Detection Limit (Analytical Sensitivi ty): 0.1 ng/mL
3. Linearity range
0.1~50 ng/ml , R≥0.990
Fast Result (15 minutes)
Simple Operation (Less Training Required)
Objective (Results Read by Analyzer)
Stringent Quality Control Insure High Accuracy
User-friendly (Simple Plug & Play Operation)
High Efficiency (Both STAT Testing and Batch Testing)
How to use?
Refer to Citest TM Fluorescence Immunoassay Analyzer Operation Manual for the complete instructions on use of the Test. The test should be in room temperature.
1. Turn on the Analyzer power. Then according to the need, select “Standard test” or “Quick test” mode.
2. Take out the ID card and insert it into the Analyzer ID Card Slot.
3. Serum or plasma: Pipette 50 μL serum or plasma into the buffer tube, mix the specimen and the buffer well.
Whole blood: Pipette 75 μL whole blood into the buffer tube; mix the specimen and the buffer well.
4. Pipette 75 μL diluted sample into the sample well of the cassette. Start the timer at the same time.
5. There are two test modes for Citest TM Fluorescence Immunoassay Analyzer, Standard Test mode and Quick Test mode.
Please refer to the user manual of Citest TM Fluorescence Immunoassay Analyzer for details.
“Quick test” mode:After 15 minutes of adding sample,Insert the test cassette into the Analyzer and click “QUICK TEST”, fill the test information and click "NEW TEST" immediately. The Analyzer will automatically give the test result after a few seconds.
“Standard test” mode:Insert the test cassette into the Analyzer immediately after adding specimen, click “STANDARD TEST”, fill the test information and click "NEW TEST" at the same time. The Analyzer will automatically countdown 15 minutes. After the countdown, the Analyzer will give the result at once.
INTERPRETATION OF RESULTS
Results read by Citest TM Fluorescence Immunoassy Analyzer.
The result of tests for PCT is calculated by Citest TM Fluorescence Immunoassay Analyzer and display the result on the screen. For additional information, please refer to the user manual of Citest TM Fluorescence Immunoassay Analyze.
Linearity range of Citest TM PCT is 0.1-50 ng/mL.
Reference range: ＜0.1ng/ml.
|PCT Test Cassette