ABP Rapid Test Cassette (Urine), AB-PINACA Rapid Test, cut-off concentration of 10ng/mL
| Principle |
Chromatographic Immunoassay |
| Format |
Dipstick, Cassette, Panel, Cup |
| Specimen |
Urine |
| Certificate |
CE |
| Reading Time |
5 minutes |
| Pack |
40T/50T |
| Storage Temperature |
2-30°C |
| Shelf Life |
2 Years |
| Sensitivity |
92.00% |
| Specificity |
97.10% |
| Accuracy |
95.80% |
| Cut-Off |
10 ng/mL* |
A rapid test for the qualitative detection of AB-PINACA in human urine.
For medical and other professional in vitro diagnostic use only.
INTENDED USE
The ABP Rapid Test Cassette (Urine) is a rapid chromatographic immunoassay for the detection of AB-PINACA in human urine at a cut-off concentration of 10ng/mL.This assay provides only a qualitative, preliminary analytical test result. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/MS) or Liquid Chromatography/mass spectrometry (LC/MS) are the preferred confirmatory methods. Clinical consideration and professional judgment should be applied to any drug of abuse test result, particularly when preliminary positive results are used.
SUMMARY
AB-PINACA is a compound that was first identified as a component of synthetic can/nabis products in Japan in 2012.1 It was originally developed by Pfizer in 2009 as an analgesic medication.2 AB-PINACA acts as a potent agonist for the CB1 receptor
(Ki = 2.87 nM, EC50 = 1.2 nM) and CB2 receptor (Ki = 0.88 nM, EC50 = 2.5 nM) and fully substitutes for Δ9-THC in rat discrimination studies, while being 1.5x more potent.
The ABP Rapid Test Cassette (Urine) is a rapid urine screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of AB-PINACA in urine. The ABP Rapid Test Cassette (Urine) yields a positive result when AB-PINACA in urine exceeds 10ng/mL.
PRINCIPLE
The ABP Rapid Test Cassette (Urine) is an immunoassay based on the principle of competitive binding. Drugs which may be present in the urine specimen compete against the drug conjugate for binding sites on the antibody. During testing, a urine specimen migrates upward by capillary action. AB-PINACA, if present in the urine specimen below 10ng/mL, will not saturate the binding sites of antibody-coated particles in the test. The antibody-coated particles will then be captured by immobilized AB-PINACA conjugate and a visible colored line will show up in the test line region.
The colored line will not form in the test line region if the AB-PINACA level exceeds 10ng/mL because it will saturate all the binding sites of anti-AB-PINACA antibodies. A drug-positive urine specimen will not generate a colored line in the test line region because of drug competition, while a drug-negative urine specimen or a specimen containing a drug concentration lower than the cut-off will generate a line in the test line region. To serve as a procedural control, a colored line will always appear in the control line region, indicating that proper volume of specimen has been added and membrane wicking has occurred.
REAGENTS
The test contains mouse monoclonal AB-PINACA antibody-coupled particles and AB-PINACA-protein conjugate. A goat antibody is employed in the control line system.
PRECAUTIONS
For medical and other professional in vitro diagnostic use only. Do not use after the expiration date.
The test should remain in the sealed pouch until use.
All specimens should be considered potentially hazardous and handled in the same manner as an infectious agent.
The used test should be discarded according to local regulations.
STORAGE AND STABILITY
Store as packaged in the sealed pouch either at room temperature or refrigerated (2-30°C). The test is stable through the expiration date printed on the sealed pouch. The test must remain in the sealed pouch until use. DO NOT FREEZE. Do not use
beyond the expiration date.
DIRECTIONS FOR USE
Allow the test, urine specimen, and/or controls to reach room temperature (15-30°C) prior to testing.
1. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it within one hour.
2. Place the test cassette on a clean and level surface. Hold the dropper vertically and transfer 3 full drops of urine (approx. 120 L) to the specimen well (S) of the test cassette, and then start the timer. Avoid trapping air bubbles in the specimen well (S). See the illustration below.
3. Wait for the colored line(s) to appear. Read results at 5 minutes. Do not interpretthe result after 10 minutes.
EXPECTED VALUES
This negative result indicates that the AB-PINACA concentration is below the detectable level of 10ng/mL. Positive result means the concentration of AB-PINACA is above the level of 10ng/mL. The ABP Rapid Test Cassette has a sensitivity of 10ng/mL.
Order Information
| Cat. No. |
Product |
Specimen |
Pack |
| DABP-102 |
AB-PINACA (ABP) Rapid Test Cassette |
Urine |
40T |
| DABP-114 |
AB-PINACA (ABP) Rapid Test Panel |
Urine |
40 T |
| DABP-101 |
AB-PINACA (ABP) Rapid Test Dipstick |
Urine |
50 T |
