Α-PVP Rapid Test Cassette (Urine), Detection Of Alpha-Pyrrolidinovalerophenone (Α-PVP)
| Principle |
Chromatographic Immunoassay |
| Format |
Dipstick, Cassette, Panel, Device, Cup |
| Specimen |
Urine,Oral Fluid,Powder |
| Certificate |
CE |
| Reading Time |
5 minutes |
| Pack |
25T/40T/50T |
| Storage Temperature |
2-30°C |
| Shelf Life |
2 Years |
| Sensitivity |
92.10% |
| Specificity |
96.80% |
| Accuracy |
95.00% |
| Cut-Off |
1000 ng/mL*, 500 ng/mL, 300 ng/mL |
α-PVP Rapid Test Cassette (Urine), detection of alpha-Pyrrolidinovalerophenone (α-PVP)
A rapid test for the qualitative detection of α-PVP in human urine.For medical and other professional in vitro diagnostic use only.
INTENDED USE
The α-PVP Rapid Test Cassette (Urine) is a rapid chromatographic immunoassay for the detection of alpha-Pyrrolidinovalerophenone (α-PVP) in human urine at a cut-off concentration of 1000ng/mL.
This assay provides only a qualitative, preliminary analytical test result. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/MS) or Liquid Chromatography/mass spectrometry (LC/MS) are the preferred confirmatory methods. Clinical consideration and professional judgment should be applied to any drug of abuse test result, particularly when preliminary positive results are used.
SUMMARY
alpha-Pyrrolidinovalerophenone (also known as α-PVP, A-PVP, alpha-PVP, and Flakka) is a synthetic stimulant substance of the cathinone and pyrrolidine chemical classes.1 α-PVP may be quantified in blood, plasma or urine to confirm a diagnosis of poisoning in hospitalized patients or to provide evidence in a medicolegal death investigation.2 It generally comes in the form of either a crystalline powder or crystallized shards which users can ingest to produce powerful but short-lived euphoric stimulant effects which are comparable to those of methamphetamine and cocaine when insufflated or vaporized.
α-PVP has been reported to be the cause, or a significant contributory cause of death in suicides and overdoses caused bycombinations of drugs.3, 4 It has also been linked to at least one death where it was combined with pentedrone and caused heart failure.
The α-PVP Rapid Test Cassette (Urine) is a rapid urine screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of alpha-Pyrrolidinovalerophenone in urine. The α-PVP Rapid Test Cassette (Urine) yields a positive result when alpha-Pyrrolidinovalerophenone in urine exceeds 1000ng/mL.
PRINCIPLE
The α-PVP Rapid Test Cassette (Urine) is an immunoassay based on the principle of competitive binding. Drugs which may be present in the urine specimen compete against the drug conjugate for binding sites on the antibody. During testing, a urine specimen migrates upward by capillary action.
alpha-Pyrrolidinovalerophenone, if present in the urine specimen below 1000ng/mL, will not saturate the binding sites of antibody-coated particles in the test. The antibody-coated particles will then be captured by immobilized alpha-Pyrrolidinovalerophenone conjugate and a visible colored line will show up in the test line region. The colored line will not form in the test line region if the alpha-Pyrrolidinovalerophenone level exceeds 1000ng/mL because it will saturate all the binding sites of anti-alpha-Pyrrolidinovalerophenone antibodies. A drug-positive urine specimen will not generate a colored line in the test line region because of drug competition, while a drug-negative urine specimen or a specimen containing a drug concentration lower than the cut-off will generate a line in the test line region. To serve as a procedural control, a colored line will always appear in the control line region, indicating that proper volume of specimen has been added and membrane wicking has occurred.
DIRECTIONS FOR USE
Allow the test, urine specimen, and/or controls to reach room temperature (15-30°C) prior to testing.
1. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it within one hour.
2. Place the test device on a clean and level surface. Hold the dropper vertically and transfer 3 full drops of urine (approx. 120 uL) to the specimen well (S) of the test cassette, and then start the timer. Avoid trapping air bubbles in the specimen well (S). See the illustration below.
3. Wait for the colored line(s) to appear. Read results at 5 minutes. Do not interpret the result after 10 minutes.
INTERPRETATION OF RESULTS
(Please refer to the illustration above)
NEGATIVE:* Two distinct colored lines appear. One colored line should be in the control line region (C), and another apparent colored line should be in the test line region (T). This negative result indicates that the alpha-Pyrrolidinovalerophenone concentrations are below the detectable level (1000ng/mL).
*NOTE: The shade of color in the test line region (T) will vary, but it should be considered negative whenever there is even a faint colored line.
POSITIVE: One colored line appears in the control region (C). No line appears in the test line region (T). This positive result indicates that the alpha-Pyrrolidinovalerophenone concentration exceeds the detectable level (1000ng/mL).
INVALID: Control line (C) fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test using a new test. If the problem persists, discontinue using the lot immediately and contact your local distributor.
QUALITY CONTROL
A procedural control is included in the test. A colored line appearing in the control region (C) is considered an internal procedural control. It confirms sufficient specimen volume, adequate membrane wicking and correct procedural technique. Control standards are not supplied with this kit; however it is recommended that positive and negative controls be tested as good laboratory practice to confirm the test procedure and to verify proper test performance.
Order Information
| Cat. No. |
Product |
Specimen |
Pack |
| DAP-102 |
α-Pyrrolidinovalerophenone (α-PVP ) Rapid Test Cassette |
Urine |
40T |
| DAP-114 |
α-Pyrrolidinovalerophenone (α-PVP ) Rapid Test Panel |
Urine |
40 T |
| DAP-101 |
α-Pyrrolidinovalerophenone (α-PVP ) Rapid Test Dipstick |
Urine |
50 T |
| DAP-802 |
α-Pyrrolidinovalerophenone (α-PVP ) Rapid Test Cassette |
Oral Fluid |
25 T |
| DAP-803 |
α-Pyrrolidinovalerophenone (α-PVP ) Rapid Test Device |
Oral Fluid |
25 T |
| DAP-X14 |
α-Pyrrolidinovalerophenone (α-PVP ) Rapid Test Panel |
Powder |
25 T |
