Carisoprodol Rapid Test Cassette (Urine), 2000ng/mL cut-off, GC/MS method, DOA rapid test
| Principle |
Chromatographic Immunoassay |
| Format |
Dipstick, Cassette, Panel, Cup |
| Specimen |
Urine |
| Certificate |
CE |
| Reading Time |
5 minutes |
| Pack |
40T/50T |
| Storage Temperature |
2-30°C |
| Shelf Life |
2 Years |
| Sensitivity |
95.00% |
| Specificity |
94.20% |
| Accuracy |
94.40% |
| Cut-Off |
2000 ng/mL |
A rapid test for the qualitative detection of Carisoprodol in human urine. For medical and other professional in vitro diagnostic use only.
INTENDED USE
The Carisoprodol Rapid Test Cassette (Urine) is a rapid chromatographic immunoassay for the detection of Carisoprodol in human urine at a cut-off concentration of 2000ng/mL.
This assay provides only a qualitative, preliminary analytical test result. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/MS) is the preferred confirmatory method. Clinical consideration and professionaljudgment should be applied to any drug of abuse test result, particularly when preliminary positive results are used.
SUMMARY
Carisoprodol, marketed under the brand name Soma among others, is a medication used for musculoskeletal pain. Use is only approved for up to three weeks. Effects generally begin within half an hour and last for up to six hours. It is taken by mouth.
Common side effects include headache, dizziness, and sleepiness. Serious side effect may include addiction, allergic reactions, and seizures. In people with a sulfa allergy certain formulations may result in problems. Safety during pregnancy and breastfeeding is not clear. Meprobamate and other muscle-relaxing drugs often were subjects of misuse in the 1950s and 60s.1,2 Overdose cases were reported as early as 1957, and have been reported on several occasions since then.
Carisoprodol is metabolized by the liver and excreted by the kidneys so this drug must be used with caution with patients that have impaired hepatic or renal function. Because of potential for more severe side effects, this drug is on the list to avoid for elderly people.
The Carisoprodol Rapid Test is a rapid urine screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of Carisoprodol in urine. The Carisoprodol Rapid Test (Urine) yields a positive result when Carisoprodol in urine exceeds 2000ng/mL.
PRINCIPLE
The Carisoprodol Rapid Test Cassette (Urine) is an immunoassay based on the principle of competitive binding. Drugs which may be present in the urine specimen compete against the drug conjugate for binding sites on the antibody.
During testing, a urine specimen migrates upward by capillary action. Carisoprodol, if present in the urine specimen below 2000ng/mL, will not saturate the binding sites of antibody-coated particles in the test. The antibody-coated particles will then be captured by immobilized carisoprodol conjugate and a visible colored line will show up in the test line region. The colored line will not form in the test line region if the Carisoprodol level exceeds 2000ng/mL because it will saturate all the binding sites of anti-meperidine antibodies.
A drug-positive urine specimen will not generate a colored line in the test line region because of drug competition, while a drug-negative urine specimen or a specimen containing a drug concentration lower than the cut-off will generate a line in the test line region.
To serve as a procedural control, a colored line will always appear in the control line region, indicating that proper volume of specimen has been added and membrane wicking has occurred.
REAGENTS
Each test line contains anti-drug mouse monoclonal antibody and corresponding drug-protein conjugates. The control line contains goat anti-rabbit IgG polyclonal antibodies and rabbit IgG.
DIRECTIONS FOR USE
Allow the test, urine specimen, and/or controls to reach room temperature (15-30ºC) prior to testing.
1. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it within one hour.
2. Place the test cassette on a clean and level surface. Hold the dropper vertically and transfer 3 full drops of urine (approx. 120 uL) to the specimen well (S) of the test cassette, and then start the timer. Avoid trapping air bubbles in the specimen well (S). See the illustration below.
3. Wait for the color line(s) to appear. The result should be read at 5 minutes. Do not interpret the result after 10 minutes.
INTERPRETATION OF RESULTS
(Please refer to the illustration)
NEGATIVE:* Two distinct colored lines appear. One colored line should be in the control line region (C), and another colored line should be in the test line region (T). This negative result indicates that the Carisoprodol concentration is below the detectable level (2000ng/mL).
*NOTE: The shade of color in the test line region (T) will vary, but it should be considered negative whenever there is even a faint colored line.
POSITIVE: One colored line appears in the control region (C). No line appears in the test line region (T). This positive result indicates that the Carisoprodol concentration exceeds the detectable level (2000ng/mL).
INVALID: Control line (C) fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test using a new test. If the problem persists, discontinue using the lot immediately and contact your local distributor.
EXPECTED VALUES
This negative result indicates that the Carisoprodol concentration is below the detectable level of 2000ng/mL.Positive result means the concentration of Carisoprodol is above the level of 2000ng/mL. The Carisoprodol Rapid Test Cassette has a sensitivity of 2000ng/mL.
Order Information
| Cat. No. |
Product |
Specimen |
Pack |
| DCAR-102 |
Carisoprodol (CAR) Rapid Test Cassette |
Urine |
40 T |
| DCAR-114 |
Carisoprodol(CAR) Rapid Test Panel |
Urine |
40 T |
| DCAR-101 |
Carisoprodol(CAR) Rapid Test Dipstick |
Urine |
50 T |
