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Ethylenediamine-Dimethylphosphinic Acid EDDP Rapid Test 5 Minutes

Ethylenediamine-Dimethylphosphinic Acid EDDP Rapid Test 5 Minutes

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    EDDP Rapid Test


    Ethylenediamine-Dimethylphosphinic Acid Rapid Test


    EDDP Drug Abuse Test Kit

  • Product Name
    Ethylenediamine-dimethylphosphinic Acid (EDDP) Rapid Test, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP)
  • Cat No.
  • Principle
    Chromatographic Immunoassay
  • Format
    Dipstick, Cassette, Panel, Cup
  • Specimen
    Urine, WB/S/P
  • Reading Time
    5 Minutes
  • Pack
  • Sensitivity
  • Specificity
  • Accuracy
  • Cut-Off
    300 Ng/mL*, 100 Ng/mL, 50 Ng/mL
  • Place of Origin
  • Brand Name
  • Certification
  • Model Number
  • Minimum Order Quantity
  • Price
  • Packaging Details
  • Delivery Time
    2-4 Weeks
  • Payment Terms
    L/C, T/T
  • Supply Ability
    10 M tests/month

Ethylenediamine-Dimethylphosphinic Acid EDDP Rapid Test 5 Minutes

Ethylenediamine-dimethylphosphinic acid (EDDP) Rapid Test, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP)


A rapid test for the qualitative detection of EDDP in human urine. For medical and other professional in vitro diagnostic use only.
Principle Chromatographic Immunoassay
Format Dipstick, Cassette, Panel, Cup
Specimen Urine, WB/S/P
Certificate CE
Reading Time 5 minutes
Pack 40T/50T
Storage Temperature 2-30°C
Shelf Life 2 Years
Sensitivity 97.90%
Specificity 99.40%
Accuracy 98.80%
Cut-Off 300 ng/mL*, 100 ng/mL, 50 ng/mL
The EDDP (Methadone Metabolite) Rapid Test Cassette (Urine) is a rapid immunochromatographic assay for the qualitative detection of 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), an inactive metabolite of methadone that acts as a narcotic pain reliever and is used as a treatment for opiate addiction.
This assay provides only a preliminary analytical test result. A more specific alternate chemical method must used in order to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/MS) is the preferred confirmatory method. Clinical consideration and professional judgment should be applied to any drug of abuse test result, particularly when preliminary positive results are used.
Methadone is an unusual drug in that its primary urinary metabolites (EDDP and EMDP) are cyclic in structure, making them very difficult to detect using immunoassays targeted to the native compound.1 Exacerbating this problem, there is a subsection of the population classified as “extensive metabolizers” of methadone. In these individuals, a urine specimen may not contain enough parent methadone to yield a positive drug screen even if the individual is in compliance with their methadone maintenance. EDDP represents a better urine marker for methadone maintenance than unmetabolized methadone.
The EDDP Rapid Test Cassette (Urine) is a rapid urine screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of EDDP in urine. The EDDP Rapid Test Cassette (Urine) yields a positive result when EDDP in urine exceeds 300 ng/mL.
The EDDP Rapid Test Cassette (Urine) is an immunoassay based on the principle of competitive binding. Drugs which may be present in the urine specimen compete against the drug conjugate for binding sites on the antibody.
During testing, a urine specimen migrates upward by capillary action. EDDP, if present in the urine specimen below 300 ng/mL, will not saturate the binding sites of antibody-coated particles in the test device. The antibody-coated particles will then be captured by immobilized EDDP conjugate and a visible colored line will show up in the test line region. The colored line will not form in the test line region if the EDDP level exceeds 300 ng/mL because it will saturate all the binding sites of anti-EDDP antibodies.
A drug-positive urine specimen will not generate a colored line in the test line region, while a drug-negative urine specimen or a specimen containing a drug concentration less than the cut-off will generate a line in the test line region. To serve as a procedural control, a colored line will always appear at the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.
The test cassette contains mouse monoclonal anti-EDDP antibody-coupled particles and EDDP-protein conjugate. A goat antibody is employed in the control line system.
  • For medical and other professional in vitro diagnostic use only. Do not use after the expiration date.
  • The test should remain in the sealed pouch until use.
  • All specimens should be considered potentially hazardous and handled in the same manner as an infectious agent.
  • The used test should be discarded according to local regulations.
Allow the test, urine specimen, and/or controls to reach room temperature (15-30ºC) prior to testing.
1. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it within one hour.
2. Place the test cassette on a clean and level surface. Hold the dropper vertically and transfer 3 full drops of urine (approx. 120 uL) to the specimen well (S) of the test cassette, and then start the timer. Avoid trapping air bubbles in the specimen well (S). See the illustration below.
Ethylenediamine-Dimethylphosphinic Acid EDDP Rapid Test 5 Minutes 0
3. Wait for the colored line(s) to appear. Read results at 5 minutes. Do not interpret the result after 10 minutes.
(Please refer to the illustration above)
NEGATIVE:* Two lines appear. One colored line should be in the control line region (C), and another apparent colored line should be in the test line region (T). A negative result indicates that the EDDP concentration is below the detectable level (300 ng/mL).
*NOTE: The shade of color in the test line region (T) may vary, but it should be considered negative whenever there is even a faint colored line.
POSITIVE: One colored line appears in the control line region (C). No line appears in the test line region (T). A positive result indicates that the EDDP concentration exceeds the detectable level (300 ng/mL). 
INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test using a new test. If the problem persists, discontinue using the lot immediately and contact your local distributor.
A procedural control is included in the test. A colored line appearing in the control line region (C) is considered an internal procedural control. It confirms sufficient specimen volume, adequate membrane wicking and correct procedural technique.
Control standards are not supplied with this kit; however, it is recommended that positive and negative controls be tested as good laboratory testing practice to confirm the test procedure and to verify proper test performance.


Order Information


Cat. No. Product Specimen Pack
DED-102 Ethylenediamine-dimethylphosphinic acid (EDDP) Rapid Test Cassette Urine 40 T
DED-114 Ethylenediamine-dimethylphosphinic acid (EDDP) Rapid Test Panel Urine 40 T
DED-101 Ethylenediamine-dimethylphosphinic acid (EDDP) Rapid Test Dipstick Urine 50 T
DED-402 Ethylenediamine-dimethylphosphinic acid (EDDP) Rapid Test Cassette WB/S/P 40 T